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cxcl10  (R&D Systems)


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    R&D Systems cxcl10
    Cxcl10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+cxcl10+protein/Recombinant+Mouse+CXCL2%2FMIP-2+Protein/pm41891183-606-80-81
    Average 94 stars, based on 31 article reviews
    cxcl10 - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Lipocalin-2 Is a Chemokine Inducer in the Central Nervous System
    Article Snippet: .. The recombinant mouse CXCL10 protein (R & D Systems) was used as a standard. ..

    Article Title: Lipocalin-2 Is a Chemokine Inducer in the Central Nervous System
    Article Snippet: CXCL10 ELISA—The levels of CXCL10 in the culture media weremeasured by sandwichELISAusing a ratmonoclonal antimouse CXCL10 antibody as the capture antibody and goat biotinylated polyclonal anti-mouse CXCL10 antibody as a detection antibody (ELISA development reagent; R & D Systems), respectively, as previously described (37). .. The recombinant mouse CXCL10 protein (R & D Systems) was used as a standard. ..

    Article Title: Inflammasome mediated neuronal-microglial crosstalk: a therapeutic substrate of the familial C9orf72 variant of frontotemporal dementia/amyotrophic lateral sclerosis
    Article Snippet: CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis MN) according to the manufacturer’s instructions. .. Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR- 608 050/CF, Minneapolis MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466- 609 NA, Minneapolis MN). .. TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.

    Article Title: Inflammasome-Mediated Neuronal-Microglial Crosstalk: a Therapeutic Substrate for the Familial C9orf72 Variant of Frontotemporal Dementia/Amyotrophic Lateral Sclerosis.
    Article Snippet: CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis, MN) according to the manufacturer’s instructions. .. Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR-608 050/CF, Minneapolis, MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466-609 NA, Minneapolis, MN). .. TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis, MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.

    Article Title: Inflammasome mediated neuronal-microglial crosstalk: a therapeutic substrate in C9orf72 -FTD/ALS
    Article Snippet: CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis MN) according to manufacturer’s instructions. .. Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR-050/CF, Minneapolis MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466-NA, Minneapolis MN). .. TNF-α and IL-1β were measured in supernatant by ELISA with Mouse TNF-alpha Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D Systems DY401, Minneapolis MN) according to manufacturer’s instructions.

    Incubation:

    Article Title: Inflammasome mediated neuronal-microglial crosstalk: a therapeutic substrate of the familial C9orf72 variant of frontotemporal dementia/amyotrophic lateral sclerosis
    Article Snippet: CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis MN) according to the manufacturer’s instructions. .. Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR- 608 050/CF, Minneapolis MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466- 609 NA, Minneapolis MN). .. TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.

    Article Title: Inflammasome-Mediated Neuronal-Microglial Crosstalk: a Therapeutic Substrate for the Familial C9orf72 Variant of Frontotemporal Dementia/Amyotrophic Lateral Sclerosis.
    Article Snippet: CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis, MN) according to the manufacturer’s instructions. .. Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR-608 050/CF, Minneapolis, MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466-609 NA, Minneapolis, MN). .. TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis, MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.

    Article Title: Inflammasome mediated neuronal-microglial crosstalk: a therapeutic substrate in C9orf72 -FTD/ALS
    Article Snippet: CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis MN) according to manufacturer’s instructions. .. Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR-050/CF, Minneapolis MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466-NA, Minneapolis MN). .. TNF-α and IL-1β were measured in supernatant by ELISA with Mouse TNF-alpha Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D Systems DY401, Minneapolis MN) according to manufacturer’s instructions.



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    a , GeneOntlogy (GO) terms up- and down-regulated in uHF-SC upon in vivo intradermal administration of LTBR-agonist compared to isotype control injected mice. b , Normalized quantification of number of CD4 + and CD8 + T cells migrated through a transwell membrane towards the supernatant collected from uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from four independent experiments. c , Normalized quantification of number of CD8 + T cells migrated through a transwell membrane towards the supernatant collected from Bulge-SC, IFE-SC or uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from three independent experiments. d , Left: Representative max projections of whole mount immunofluorescence of the bulge, uHF and IFE compartment of wild type mice showing distribution of CD4 + cells (in white). Bulge cells are marked by Krt24 in green and upper hair follicle cells are marked by LRIG1 in light blue. Right: Quantification of the average distance of CD4 + and CD8 + T cells from Krt24 and LRIG1 quantified upon surface rendering, n =2 mice. e , qPCR analysis of <t>Cxcl10</t> and Cxcl16 expression Bulge-SC, uHF-SC and IFE-SC FACS-sorted from wild type mice. Each dot represents one mouse from two independent experiments. f , Representative images of MSA plates showing bacterial colonies grown after swabbing the back skin of Ltb-CreER x R26-DTA and R26-DTA (ctrl) mice associated with S. epidermidis . Data in b and d are analysed by unpaired two-tailed Student’s t- test. Data in c and e are analysed by ordinary one-way ANOVA with Tukey’s multiple comparison post-test. p -values are indicated in each figure and data are represented as mean with SEM.
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    a , GeneOntlogy (GO) terms up- and down-regulated in uHF-SC upon in vivo intradermal administration of LTBR-agonist compared to isotype control injected mice. b , Normalized quantification of number of CD4 + and CD8 + T cells migrated through a transwell membrane towards the supernatant collected from uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from four independent experiments. c , Normalized quantification of number of CD8 + T cells migrated through a transwell membrane towards the supernatant collected from Bulge-SC, IFE-SC or uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from three independent experiments. d , Left: Representative max projections of whole mount immunofluorescence of the bulge, uHF and IFE compartment of wild type mice showing distribution of CD4 + cells (in white). Bulge cells are marked by Krt24 in green and upper hair follicle cells are marked by LRIG1 in light blue. Right: Quantification of the average distance of CD4 + and CD8 + T cells from Krt24 and LRIG1 quantified upon surface rendering, n =2 mice. e , qPCR analysis of <t>Cxcl10</t> and Cxcl16 expression Bulge-SC, uHF-SC and IFE-SC FACS-sorted from wild type mice. Each dot represents one mouse from two independent experiments. f , Representative images of MSA plates showing bacterial colonies grown after swabbing the back skin of Ltb-CreER x R26-DTA and R26-DTA (ctrl) mice associated with S. epidermidis . Data in b and d are analysed by unpaired two-tailed Student’s t- test. Data in c and e are analysed by ordinary one-way ANOVA with Tukey’s multiple comparison post-test. p -values are indicated in each figure and data are represented as mean with SEM.
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    Image Search Results


    a , GeneOntlogy (GO) terms up- and down-regulated in uHF-SC upon in vivo intradermal administration of LTBR-agonist compared to isotype control injected mice. b , Normalized quantification of number of CD4 + and CD8 + T cells migrated through a transwell membrane towards the supernatant collected from uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from four independent experiments. c , Normalized quantification of number of CD8 + T cells migrated through a transwell membrane towards the supernatant collected from Bulge-SC, IFE-SC or uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from three independent experiments. d , Left: Representative max projections of whole mount immunofluorescence of the bulge, uHF and IFE compartment of wild type mice showing distribution of CD4 + cells (in white). Bulge cells are marked by Krt24 in green and upper hair follicle cells are marked by LRIG1 in light blue. Right: Quantification of the average distance of CD4 + and CD8 + T cells from Krt24 and LRIG1 quantified upon surface rendering, n =2 mice. e , qPCR analysis of Cxcl10 and Cxcl16 expression Bulge-SC, uHF-SC and IFE-SC FACS-sorted from wild type mice. Each dot represents one mouse from two independent experiments. f , Representative images of MSA plates showing bacterial colonies grown after swabbing the back skin of Ltb-CreER x R26-DTA and R26-DTA (ctrl) mice associated with S. epidermidis . Data in b and d are analysed by unpaired two-tailed Student’s t- test. Data in c and e are analysed by ordinary one-way ANOVA with Tukey’s multiple comparison post-test. p -values are indicated in each figure and data are represented as mean with SEM.

    Journal: bioRxiv

    Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis

    doi: 10.64898/2026.01.28.701831

    Figure Lengend Snippet: a , GeneOntlogy (GO) terms up- and down-regulated in uHF-SC upon in vivo intradermal administration of LTBR-agonist compared to isotype control injected mice. b , Normalized quantification of number of CD4 + and CD8 + T cells migrated through a transwell membrane towards the supernatant collected from uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from four independent experiments. c , Normalized quantification of number of CD8 + T cells migrated through a transwell membrane towards the supernatant collected from Bulge-SC, IFE-SC or uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from three independent experiments. d , Left: Representative max projections of whole mount immunofluorescence of the bulge, uHF and IFE compartment of wild type mice showing distribution of CD4 + cells (in white). Bulge cells are marked by Krt24 in green and upper hair follicle cells are marked by LRIG1 in light blue. Right: Quantification of the average distance of CD4 + and CD8 + T cells from Krt24 and LRIG1 quantified upon surface rendering, n =2 mice. e , qPCR analysis of Cxcl10 and Cxcl16 expression Bulge-SC, uHF-SC and IFE-SC FACS-sorted from wild type mice. Each dot represents one mouse from two independent experiments. f , Representative images of MSA plates showing bacterial colonies grown after swabbing the back skin of Ltb-CreER x R26-DTA and R26-DTA (ctrl) mice associated with S. epidermidis . Data in b and d are analysed by unpaired two-tailed Student’s t- test. Data in c and e are analysed by ordinary one-way ANOVA with Tukey’s multiple comparison post-test. p -values are indicated in each figure and data are represented as mean with SEM.

    Article Snippet: When indicated, 50μg/ml of InVivoMab anti-mouse CXCL10 (IP-10, BioXcell) was added to the lower chamber.

    Techniques: In Vivo, Control, Injection, Membrane, Immunofluorescence, Expressing, Two Tailed Test, Comparison